ON-SITE RAPID SCREENING FOR SUGARCANE SMUT RESISTANCE USING NEAR-INFRARED (NIR) SPECTROSCOPY
By DE PURCELL; JP OXLEY; MC COX; BJ CROFT; MG O’SHEA
THIS PAPER describes the process developed for a rapid, non-destructive,
on-site screening technique based on NIR spectroscopy for rating the
resistance of sugarcane clones to sugarcane smut. Upon implementation,
this technique will allow the screening of larger plant populations at
earlier stages within the selection program, thereby realising considerable
gains in efficiency and a significant retention of genetic variability. Thirty
one blind sugarcane samples were selected for a validation trial conducted
in Bundaberg during the 2009 season. The NIR spectra of stalk bud tissue
were collected, pretreated and submitted for chemometric analysis. The
NIR-predicted smut ratings were compared to the traditionally derived
field trial values, with very encouraging results for the development of an
early screening tool.